TEM and SEM

Sample preparation

* Prepare your stock solution of sample (at about 1mg/mL), some Parafilm, the supports (copper grids for TEM and 8x22mm glass plates for SEM) and a pair of small clean Brussel stainless steel tweezers.

* Place one support on the Parafilm (for the grids, the copper side have to be oriented upwardly) and deposit a drop of 15 microL on it for 1 to 2 minutes.

* Dry the sample with a piece of blotting paper.

* Place the sample under the hood for at least 30 minutes.

* Clean the grid/glass plate with 300 microL of milli-Q water and then dry the sample with blotting paper. This step is particularly important to remove the salts coming with the buffer.

* Dry the sample under the hood for at least 30 minutes.

Upper image : The Parafilm sheet, the grids and a pair of Brussel stainless steel tweezers needed for preparing the TEM samples.



Upper image : The grid on the Parafilm (the copper side have to be oriented upwardly).



Upper image : The deposition of 15 microL on the grid.



Upper image : Removing the buffer with blotting paper.



Upper image : The washing of the grid with 300 microL of milli-Q water.