ssNMR

Peptide into oriented membranes (on glass plates)

* Weight 50 mg of lipids and some mg of peptides to the desired P/L ratio (P/L ≈ 1 to 2% mol/mol). Put the mixture of powder in a test tube.

* Dissolve the mixture with some mL of appropriate solvents (this can include chloroform, methanol, TFE, HFIP, DMSO).

* Concentrate the solution under a flow of nitrogen until it becomes viscus (some hundreds of microL). The solution have to remains transparent and free of aggregates.

* Deposit some droplets (some microL) of sample onto 20 micro glass plates (8 x 11 mm).

* Place the 20 micro plates under vacuum for about 24 hours in order to fully remove the solvents from the sample.

* Place the sample in a closed chamber at 93% relative humidity for at least 48 hours.

* Stack the glass plates on top of each others. Wrap the sample in Teflon strips and slide it into a micro plastic bag.

Upper image : 50mg of lipids and some mg of peptides in a test tube.



Upper image : Solubilization of the lipids and the peptides in some mL of solvent.



Upper image : Concentration of the sample to some hundreds of microL by evaporation of the solvent under nitrogen flow (note that the solution remains transparent).



Upper image : Deposition of some microL of solution of the mixture onto 20 micro glass plates (note that the deposition remains transparent, even after evaporation of the solvent).



Upper image : Stack of 20 glass plates wrapped into Teflon strips and packed in a micro plastic bag.