Circular Dichroism

Buffer Requirements

* Suitable buffer ions for UV studies in the range of 260 to 190 nm include phosphate, Tris, acetate and borate. These buffers are suitable for pH values ranging from 5 to 10 and should be chosen in accordance to your needs.

* 10 mM of buffer is generally a good starting point if you plan to work with cuvettes of 1 mm long. For longer path cuvettes the concentration of the buffer should be reduced in accordance.

Sample Requirements

* The sample should be solubilized and as pure as possible to avoid experimental artifacts.
* The sample concentration is determined by the pathlength of the cuvette. 0.1 mg/ml of peptide or protein is a good starting point to work with 1 mm long cuvette.
* Sample volume is function of the geometry of the cuvette : 200 microL is the minimum volume needed to work with the 1 mm long cuvettes.
* Working with thinner cuvettes and highly concentrated sample is possible and can help to reduce contribution of the buffer in the spectra.

Upper image : filling the 1mm long cuvette with 200microL of sample solution at 0.1mg/ml



Upper image : picture of the cuvette holder of the Jasco J-810

Working with Vesicles and Micelles

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